• Pb(Ⅱ)胁迫培养下Pseudomonas putida EPS组分变化及其对铅的吸附性能
  • Changes of EPS components of Pseudomonas putida under Pb(Ⅱ) stress and its adsorption performance for lead
  • 基金项目:国家重点研发计划(No. 2018YFD0800700);广东高校省级重点平台和重大科研项目(No. 2017GKZDXM007);广东高校省级重点平台和重大科研项目(No. 2017GKCXTD004)
  • 作者
  • 单位
  • 曾刘婷
  • 广东工业大学环境科学与工程学院
  • 杨贤
  • 广东工业大学环境科学与工程学院
  • 廖小健
  • 广东工业大学环境科学与工程学院
  • 李寿朋
  • 广东工业大学环境科学与工程学院
  • 关智杰
  • 广东工业大学环境科学与工程学院
  • 孙水裕
  • 广东工业大学环境科学与工程学院
  • 方小弟
  • 广东工业大学环境科学与工程学院
  • 摘要:近年来,由微生物产生的胞外聚合物(Extracellular Polymeric Substances,EPS)对重金属废水的处理技术引起人们的关注。本研究采用Pb(Ⅱ)胁迫培养恶臭假单胞菌(Pseudomonas putida,P. putida),定向调控EPS的化学组成,增加EPS产量,并提高EPS对Pb(Ⅱ)的吸附性能。结果表明,Pb(Ⅱ)胁迫浓度为10 mg/L时,P. putida EPS产量最高,达到131.55 mg/g VSS,较胁迫前增加25.35%;其中蛋白质含量显著增加,达到87.01 mg/g VSS,较胁迫前增加35.15%。在此条件下,EPS对Pb(Ⅱ)的吸附量也达到最大值,为1372.95 mg/g。3D-EEM、FTIR结果表明,胁迫后EPS中氨基酸和蛋白质类物质以及C=O、N-H、-COO-、C-N、-OH等负电性官能团增加。进一步实验结果表明,吸附实验最佳pH为6.0,吸附过程符合拟二级动力学和Freundlich等温模型。胁迫后P. putida EPS对Pb(Ⅱ)的理论吸附容量为1452.59 mg/g,较胁迫前提高74.05%。本研究可为制备高效的生物吸附剂提供一定的理论基础。
  • Abstract:In recent years, the treatment technology of heavy metal wastewater by extracellular polymeric substances (EPS) produced by microorganisms has attracted people's attentions. Herein, Pseudomonas putida (P. putida) was cultured under Pb(Ⅱ) stress to regulate the chemical composition of EPS directionally, increase the yield of EPS, and improve the adsorption performance of EPS to Pb(Ⅱ). The results showed that the maximum yield of P. putida EPS and protein content reached to 131.55 and 87.01 mg/g VSS respectively when the Pb(Ⅱ) stress concentration was 10 mg/L, which were 25.35 and 35.15% higher than that before stress respectively. The adsorption capacity of Pb(Ⅱ) by EPS also reached the maximum of 1372.95 mg/g under this condition. According to the 3D-EEM and FTIR results, it illustrated that amino acids, protein substances and electronegative functional groups such as C=O, N-H, -COO-, C-N, -OH in EPS increased after stress. Further experimental results showed that the optimal pH of the adsorption experiment was 6.0, and the adsorption process conformed to the pseudo-second-order kinetics and Freundlich isotherm model. The theoretical adsorption capacity of P. putida EPS for Pb(Ⅱ) was 1452.59 mg/g after stress, which was 74.05% higher than that before stress. This research can provide a certain theoretical basis for the preparation of high-efficiency biosorbents.

  • 摘要点击次数: 35 全文下载次数: 0