• 章豪,冯鑫,单捷,潘杨.聚磷生物膜反应器磷负荷提升过程中微生物种群分析[J].环境科学学报,2019,39(11):3764-3771

  • 聚磷生物膜反应器磷负荷提升过程中微生物种群分析
  • Microbial community analysis in a phosphorus biofilm reactor during enhanced phosphorus loading
  • 基金项目:国家自然科学基金(No.51778390);国家重点研发计划项目(No.2016YFC0401108);江苏省自然科学基金(No.BK20171219)
  • 作者
  • 单位
  • 章豪
  • 1. 苏州科技大学环境科学与工程学院, 苏州 215009;2. 苏州科技大学环境生物技术研究所, 苏州 215009
  • 冯鑫
  • 1. 苏州科技大学环境科学与工程学院, 苏州 215009;2. 苏州科技大学环境生物技术研究所, 苏州 215009
  • 单捷
  • 1. 苏州科技大学环境科学与工程学院, 苏州 215009;2. 苏州科技大学环境生物技术研究所, 苏州 215009
  • 潘杨
  • 1. 苏州科技大学环境科学与工程学院, 苏州 215009;2. 苏州科技大学环境生物技术研究所, 苏州 215009;3. 江苏省环境科学与工程重点实验室, 苏州 215009
  • 摘要:在同步去除及富集磷酸盐的基础上,通过研究水力停留时间(8 h、6 h、4 h)及不同的进水磷浓度考察磷负荷对于反应器的运行效能和微生物群落结构的影响,探究生物膜反应器所能承受的最大磷负荷,并探究微生物种群与工艺性能的响应关系,获取高效生物膜驯化的最优进水磷条件,以及分析该条件下的种群结构.结果表明,在驯化阶段,在磷负荷低于0.18 kg·m-3·d-1时,磷负荷的提升不会影响磷去除率,磷去除率保持在98.3%;当磷负荷达到0.24 kg·m-3·d-1时,磷去除率下降到84.3%,但Prel/Cupt依旧从最初的0.06上升至0.121.MiSeq测序结果表明优势菌门为变形菌门(Proteobacteria),从59.2%增长至83.5%,反应器中的优势聚磷菌科为红环菌科(Rhodocyclaceae),从11.8%增长至27.3%.在回收阶段,在保证好氧出水达标的情况下,磷酸盐浓度升高至56.4 mg·L-1,富集液浓度达到鸟粪石法回收磷的标准.通过增加进水磷负荷可使聚磷菌丰度提高,进而提高了回收液磷浓度.
  • Abstract:On the basis of simultaneous removal and enrichment of phosphate, this experiment investigated the operational efficacy of the reactor and the microbial community structure under different hydraulic retention time (8 h, 6 h, 4 h) and influent phosphorus concentrations, to explore the maximum phosphorus load that a biofilm reactor can withstand and the response of microbial populations to different conditions for efficient biofilm domestication. The results show that during the acclimation stage, with the phosphorus load increase within 0.18 kg·m-3·d-1, the phosphorus removal rate remained at 98.3%, suggesting that the increase of phosphorus load did not affect the phosphorus removal rate. On the contrary, the phosphorus removal dropped to 84.3%, but the Prel/Cupt still rose from 0.06 to 0.121 under the conditions of the phosphorus load with 0.24 kg·m-3·d-1. MiSeq sequencing resluts show that the dominant phylum was Proteobacteria, which increased from 59.2% to 83.5%. The dominant polyphosphate family in the reactor was Rhodocyclaceae, which increased from 11.8% to 27.3%. In the recovery stage, the phosphorus-rich concentrate is raised to 56.4 mg·L-1, and the concentration of the recovered liquid reaches the standard for recovering phosphorus by the struvite method, ensuring that the aerobic effluent reaches the standard. when increasing the influent phosphorus load, the population abundance of the polyphosphate bacteria increased, then increasing the phosphorus concentration of the recovered liquid.

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