Abstract：Bioturbation of benthic animals at the sediment-water interface plays an important role in microbial denitrification and anammox processes. In this study， we compared the effects of two macroinvertebrates with different disturbance functions （Branchiura sowerbyi and Radix swinhoei） on denitrification and anammox and nitrogen （N） removal with varying nitrogen load levels. The results showed that the abundance of denitrifying bacteria in the sediments increased by 1.61~3.48 times， and the abundance of anammox bacteria increased by 1.53~2.89 times. Nitrogen removal in the system promoted by B. sowerbyi and R. swinhoei increased by 16.64~32.18 mg and 15.34~39.25 mg， respectively. The nitrogen removal in system 2 is higher than that in system 1. The perturbation of the B. sowerbyi promoted the concentration of nitrogen （NH4+-N， NO3--N and TN） in the overlying water to increase by 17.67%~230.54%. In contrast， R. swinhoei had no significant effect on the concentrations of NO3--N and TN in the overlying water. In systems 1 and 2， the abundance of anammox bacteria in the R. swinhoei group was higher compared to that in the B. sowerbyi group， and the relative abundance of the anammox bacteria affiliated phylum Planctomycetes increased by 10.00%~15.12%. The results can provide a reference and basis for alleviating nitrogen pollution in freshwater ecosystems and restoring eutrophic water bodies.