Abstract：Excitation emission matrix spectroscopy， synchronous fluorescence spectroscopy and resin isolation were used to characterize the DOM in runoff from urban road， roof， square and grassland in Beijing. The results showed that the concentrations， substances composition and chemical fractions of DOM were affected by the landcovers. The DOC concentration in initial runoff from road and roof were relatively high and decreased with the increasing runoff duration， while the DOC concentration in runoff from grassland increased with the runoff duration and it maintained a low level in runoff from the square. As a result of the traffic activities and the atmospheric deposition， DOM in runoff from road， roof and square were mainly composed of fulvic acid， aromatics， and proteinaceous substances. And hydrophobic organics， especially neutral and acidic hydrophobic organics， were the dominant fractions in DOM in runoff from these landcovers. In addition to fulvic-acid and aromatic proteinaceous substances， it also contained a large amount of humic substances and biogenic proteinaceous substances in DOM in runoff from grassland due to the soil organic matter scouring. However， hydrophilic organics， especially the neutral and acidic hydrophilic organics， were the dominant fractions in DOM in runoff from grassland.