Abstract：The treatment and reuse of greywater is the key to guarantee the efficient water cycle in the controlled ecological life support system （CELSS）. In this study， anaerobic membrane bioreactor （AnMBR） was applied to treat greywater after urine was source separated in CELSS， the treatment efficiency and microbial community succession were investigated. It was found that the main dissolved organic matter （DOM） in the greywater is tryptophan， surfactants， and fulvic acid， and the COD removal rate was 65%~75% with a high surfactants removal efficiency of 80%. The issue of surfactants accumulation in AnMBR can be significantly solved， but the high temperature and low HRT would restrain the degradation of surfactants， and the COD removal efficiency was decreased accordingly. The high-throughput sequencing study reveals that the microbial related to surfactants degradation was the key， and Synergistes， Citrobacter were recognized as the key genus. But the high temperature and low HRT would restrain the degradation activity of microbes，， which would in turn influence the microbial community in AnMBR. The key genus turned from Methanosarcina， Syntrophomonas， Keratinibaculum， which were mainly related to methane metabolism， to Proteiniclasticum， Pseudomonas， Aminobacterium， Citrobacter，Desulfovibrio. Methanogens， especially for Methanosarcina， were significantly inhibited. Sulfate reduction bacteria and the acidogenic bacteria were enhanced， lead to the increase consumption of alkalinity. As a result， the EPS， MLSS， and particle size of sludge were decreased， the sludge production was significantly low， which benefit the application in space-limited condition. In conclusion， AnMBR is a potential substitute for greywater treatment in CELSS， which could promote the improvement of deep-space exploration. However， the loss of sludge and the accumulation of surfactants need further investigation to ensure the stability of removal efficiency.