研究报告

  • 张红娜,常美洁,刘敬博,董梦洁,孙佳欣,翟真真.基于病毒宏基因组学鉴定水环境中噬菌体携带的新型耐药基因及其耐药特点分析[J].环境科学学报,2022,42(6):215-224

  • 基于病毒宏基因组学鉴定水环境中噬菌体携带的新型耐药基因及其耐药特点分析
  • Identifying novel phage-harbored antibiotic resistance genes in water environments by viral metagenome and analyzing their antibiotic resistance profile
  • 基金项目:国家自然科学基金项目(No.81903282);河北省省级大学生创新创业训练计划项目(No.S202011832011);河北省自然科学基金项目(No.C2020207012);山东省医药卫生科技发展计划项目(No.202012070823)
  • 作者
  • 单位
  • 张红娜
  • 河北经贸大学生物科学与工程学院,石家庄 050061
  • 常美洁
  • 河北经贸大学生物科学与工程学院,石家庄 050061
  • 刘敬博
  • 山东农业大学动物科技学院,泰安 271000
  • 董梦洁
  • 河北经贸大学生物科学与工程学院,石家庄 050061
  • 孙佳欣
  • 河北经贸大学生物科学与工程学院,石家庄 050061
  • 翟真真
  • 泰安市中心医院,泰安 271000
  • 摘要:水环境作为耐药基因的存储库,为耐药基因的水平转移和新型耐药基因的产生提供重要场所.但到目前为止,对水环境中病毒携带耐药基因的特征及其功能还知之甚少.因此以南四湖和东平湖为研究对象,利用病毒宏基因组学结合体外实验的方法探讨水环境中病毒携带新型耐药基因的情况及其耐药特点.通过分析病毒基因组的片段(reads)和重叠群(contigs),鉴定出多种耐药基因,包括AAC(6’)、QnrA、VanY、Vatβ-内酰胺酶基因.值得关注的是,通过核心序列比对和进化分析,挖掘出两种噬菌体携带的新型β-内酰胺酶基因blaNSDPV-1blaNSVM-1.最小抑菌浓度试验表明,这两种编码新型β-内酰胺酶的耐药基因对临床常用头孢类和碳青霉烯类抗生素具有一定的耐药性.综上所述,水环境中噬菌体很可能在新型耐药基因的产生和耐药基因散播中起着重要的作用.另外,病毒宏基因组学结合体外实验是发现噬菌体携带新型耐药基因的重要方法.
  • Abstract:As a pivotal reservoir of antibiotic resistance genes (ARGs), water environment has become an ideal place for the spread of ARGs and generation of new ARGs. But until now, information about characteristics and functions of phage-borne ARGs in aquatic environments is poorly defined. Here viral metagenomics was used to investigate novel phage-brone ARGs in Nansi Lake and Dongping Lake and then the antibiotic resistance profile of novel phage-borne ARGs was determined by in vitro experiments. The results showed that different classes of ARGs were identified from viromes through analyzing viral reads and contigs, including AAC(6'), QnrA, VanY, Vat, and β-lactamase genes. More importantly, two novel phage-carried genes encoding β-lactamase, blaNSDPV-1 and blaNSVM-1, were identified by sequence analysis, and the minimum inhibitory concentration test showed that both blaNSDPV-1 and blaNSVM-1 displayed resistance to cephalosporins and carbapenems. In conclusion, these findings indicate that water-borne phages are a reservoir of ARGs or even novel ARGs, therefore playing an important role in the dissemination of ARGs. In addition, viral metagenomics combined with in vitro experiments is an important method to discover novel phage-borne ARGs.

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