Abstract：Organic pollutants of wastewater in industrial parks are generally complex， highly toxic and difficult to remove， which may still be potential ecotoxic effects after treatment. Therefore， it is of great significant to evaluate the treatment efficiency of wastewater in the industrial parks. In this study， zebrafish embryo test and non-target contaminant screening method based on liquid chromatography-mass spectrometry（LC-MS） were used to analyze the treatment efficiency of two typical industrial park wastewater treatment plants. The combination of biological endpoints and chemical analyses contributed to explaining the changes of toxic pollutants in wastewater along the treatment process. results demonstrated that the biochemical treatment process could not effectively remove zebrafish embryotoxic substances in industrial wastewater. The advanced oxidation processes， adsorption process， and membrane bio-reacter（MBR） based process further eliminated pollutants and their toxicities. The combination of municipal and industrial wastewater enhanced the removal of toxic pollutants in industrial wastewater and reduced biological toxicity. Overall， it is feasible to combine zebrafish embryotoxicity and non-target chemical screening to determine the efficiency of industrial wastewater treatment， which is helpful in guiding the design and regulation of the industrial wastewater treatment process.