研究报告

  • 姚雪薇,邵贤明,王康舟,吕恺,彭党聪.不同基质条件下厌氧氨氧化SMBBR启动特性研究[J].环境科学学报,2021,41(10):4049-4055

  • 不同基质条件下厌氧氨氧化SMBBR启动特性研究
  • Anammox SMBBR fast start-up with different substrates
  • 基金项目:陕西省重点研发计划(No.2019ZDLSF06-05)
  • 作者
  • 单位
  • 姚雪薇
  • 西安建筑科技大学环境与市政工程学院, 西安 710055
  • 邵贤明
  • 西安建筑科技大学环境与市政工程学院, 西安 710055
  • 王康舟
  • 西安建筑科技大学环境与市政工程学院, 西安 710055
  • 吕恺
  • 西安建筑科技大学环境与市政工程学院, 西安 710055
  • 彭党聪
  • 1. 西安建筑科技大学环境与市政工程学院, 西安 710055;2. 西北水资源与环境生态教育部重点实验室, 西安 710055
  • 摘要:通过接种某城市污水处理厂好氧池生物膜,采用NH4+-N+NO2--N (SMBBR-1)和NH4+-N+NO3--N+HAc (SMBBR-2)两种进水基质启动厌氧氨氧化序批式移动床生物膜反应器(Sequencing Moving Bed Biofilm Reactor,SMBBR),研究不同基质条件下反应器的启动特性.结果表明,两反应器在运行100 d后均成功启动并稳定运行,在进水负荷分别为0.83和0.32 kg·m-3·d-1(以N计)的条件下,氮去除率分别达到81.82%±1.20%和66.35%±4.79%.活性测定结果显示,SMBBR-1和SMBBR-2中Anammox活性分别达到6448.32和1980.32 mg·m-2·d-1,表明Anammox菌被成功富集.高通量结果显示,SMBBR-1和SMBBR-2中启动成功后的Anammox菌由Ca.BrocadiaCa.Jettenia组成,其中,Ca.Brocadia占比分别为11.02%和7.57%,Ca.Jettenia占比分别为2.07%和0.56%.除Anammox菌外,SMBBR-2中还包括Thauera(2.84%)和Flavobacterium菌(0.66%),其为部分反硝化菌的主导菌属.本研究表明,虽然两种不同基质的启动办法各有利弊,但其均能实现厌氧氨氧化SMBBR的启动,可为主流系统内的Anammox菌快速富集培养提供技术支撑.
  • Abstract:To investigate the characteristics of anammox enrichment with different substrates, two SMBBRs with aerobic biofilm taken from municipal wastewater treatment were established. One was fed with ammonium and nitrite (SMBBR-1), the other was fed with ammonium,nitrate and acetic acid (SMBBR-2). The long-term operation results showed 81.82%±1.20% and 66.35%±4.79% of nitrogen removal efficiencies were stably obtained under the nitrogen loading rate (NLR) of 0.83 and 0.32 kg·m-3·d-1 for SMBBR-1 and SMBBR-2 after 100 days' operation. The anammox activities of SMBBR-1 and SMBBR-2 could reach 6448.32 and 1980.32 mg·m-2·d-1, respectively, which suggested that anammox bacteria were successfully enriched in two SMBBRs. The high-throughput sequencing results revealed Ca. Brocadia and Ca. Jettenia were the predominance for anammox bacteria in SMBBR-1 and SMBBR-2. The relative abundance of Ca. Brocadia were 11.02% and 7.57%, and the abundance of Ca. Jettenia were 2.07% and 0.56%, respectively. The Thauera and Flavobacterium were also detected with relative abundance of 2.84% and 0.66% in SMBBR-2, which were the dominated species of partial denitrifying bacteria. This study showed that enrichment methods with different substrates for anammox SMBBR start-up had the advantages and disadvantages. However, these two substrates strategies could be used for reactor establishment and provided support for anammox enrichment in mainstream.

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