• 滕晓强,陆宏峰,郭心月,高畅,罗钦方,张瑜,洪华嫦,孙洪杰.二氯苯醌对斑马鱼胚胎发育及超氧化物歧化酶的影响[J].环境科学学报,2020,40(7):2659-2664

  • 二氯苯醌对斑马鱼胚胎发育及超氧化物歧化酶的影响
  • The effect of 2,6-DCBQ on the development and superoxide dismutase of zebrafish embryo
  • 基金项目:国家自然科学基金(No.21707123);浙江省公益应用项目(No.LGF19B070007)
  • 作者
  • 单位
  • 滕晓强
  • 浙江师范大学地理与环境科学学院, 金华 321004
  • 陆宏峰
  • 浙江师范大学地理与环境科学学院, 金华 321004
  • 郭心月
  • 浙江师范大学地理与环境科学学院, 金华 321004
  • 高畅
  • 浙江师范大学地理与环境科学学院, 金华 321004
  • 罗钦方
  • 浙江师范大学地理与环境科学学院, 金华 321004
  • 张瑜
  • 浙江师范大学地理与环境科学学院, 金华 321004
  • 洪华嫦
  • 浙江师范大学地理与环境科学学院, 金华 321004
  • 孙洪杰
  • 浙江师范大学地理与环境科学学院, 金华 321004
  • 摘要:2,6-二氯-1,4-苯醌(2,6-dichloro-1,4-benzoquinone,2,6-DCBQ)作为一种新型消毒副产物,因其高检出率及高含量的特点而备受人们关注.本研究以斑马鱼胚胎为实验材料探究了2,6-DCBQ的发育毒性.结果发现:斑马鱼胚胎在8~24 hpf(器官发育形成时期)对2,6-DCBQ暴露最为敏感,死亡率最高;2,6-DCBQ安全浓度为27.15 μg·L-1,属于极度危险外源化学物.在本实验中,2,6-DCBQ对斑马鱼的生长没有显著影响,但高浓度(100和150 μg·L-1)2,6-DCBQ会显著增加斑马鱼胚胎及仔鱼的畸形率.在实验过程中还发现,高浓度(≥ 80 μg·L-1)2,6-DCBQ会明显抑制斑马鱼胚胎的心率.此外,≥ 30 μg·L-1 2,6-DCBQ可提高超氧化物歧化酶(Superoxide dismutase,SOD)的活性,并影响Cu/ZnSOD和MnSOD的正常转录.本研究较全面地探究了2,6-DCBQ对斑马鱼早期发育阶段的发育毒性,可为2,6-DCBQ的毒性研究提供可靠证据.
  • Abstract:2,6-dichloro-1,4-benzoquinone (2,6-DCBQ), as an emerging disinfection by-products, has attracted much attentions due to frequently detected and high levels. In this study, zebrafish embryo was employed to explore the toxic effect of 2,6-DCBQ on the developmental performance of zebrafish embryo. Our results clearly indicated that zebrafish embryo at 8~24 hpf was most sensitive to 2,6-DCBQ, and more embryos died at 8~24 hpf. The safe concentrations of 2,6-DCBQ was 27.15 μg·L-1, and thus 2,6-DCBQ is classified as the extremely dangerous pollutant. In the present study, 2,6-DCBQ had no significant effect on zebrafish growth, whereas higher concentrations (100 and 150 μg·L-1) of 2,6-DCBQ obviously inhibited zebrafish embryo heart beating. Besides, in this study, over 30 μg·L-1 of 2,6-DCBQ significantly elevated superoxide dismutase (SOD) activity and disrupted the transcriptional levels of Cu/ZnSOD and MnSOD. Our results comprehensively demonstrate the toxic effect of 2,6-DCBQ on developmental performance of zebrafish embryos, providing reliable evidence for 2,6-DCBQ toxicity.

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