研究报告

  • 韩彦莎,杨浩,仪慧兰.外源脱落酸缓解二氧化硫对胡杨细胞的毒性[J].环境科学学报,2018,38(7):2948-2954

  • 外源脱落酸缓解二氧化硫对胡杨细胞的毒性
  • Exogenous abscisic acid alleviates sulfur dioxide-induced toxicity in Populus euphratica cells
  • 基金项目:国家自然科学基金(No.31500504,31371868);山西省高等学校科技创新项目(No.2017102)
  • 作者
  • 单位
  • 韩彦莎
  • 山西大学生命科学学院, 太原 030006
  • 杨浩
  • 山西大学生命科学学院, 太原 030006
  • 仪慧兰
  • 山西大学生命科学学院, 太原 030006
  • 摘要:二氧化硫(SO2)是一种常见的大气污染物.前期研究表明,过氧化氢(H2O2)和一氧化氮(NO)参与调控SO2诱导的胡杨细胞毒性.脱落酸(ABA)是一种植物逆境激素,目前关于ABA在植物细胞响应SO2胁迫中的具体作用并不清楚.因此,本文以胡杨愈伤细胞为材料,采用SO2衍生物处理,研究外源ABA在植物细胞SO2毒性缓解中的作用及相关机制.结果发现:2 mmol·L-1 SO2衍生物处理胡杨细胞9 h后,细胞电解质外渗率和细胞死亡率明显增加;外源施用5 μmol·L-1 ABA可明显缓解SO2对胡杨细胞的毒性,降低电解质外渗率和细胞死亡率.2 mmol·L-1 SO2衍生物胁迫下,与SO2单独处理组相比,添加外源ABA能够使胡杨细胞的抗坏血酸过氧化物酶(APX)和过氧化氢酶(CAT)活性分别提高23.9%和48.0%,从而减少胞内H2O2水平.此外,SO2胁迫下,外施ABA后胡杨细胞的PeNia1、PeNia2基因表达受到明显抑制,细胞硝酸还原酶(NR)活性降低24.1%,胞内NO合成减少.以上结果表明,SO2胁迫下,外源施加ABA可有效降低胡杨细胞内H2O2和NO水平,从而缓解SO2对胡杨细胞的毒性.
  • Abstract:Sulfur dioxide (SO2) is a common air pollutant. Previous studies revealed that hydrogen peroxide (H2O2) and nitric oxide (NO) regulated SO2-induced cell toxicity of Populus euphratica. Abscisic acid (ABA) is a phytohormone involved in plant response to abiotic stress. To date, the role of ABA in cell responses to SO2 stress is largely unknown. In the present study, using SO2 derivatives treatment, we investigated the relevant mechanisms of exogenous ABA alleviation of SO2-induced toxicity in P. euphratica callus cells. The results showed that 2 mmol·L-1 SO2 derivatives treatment (9 h) caused significant increase of cell death and electrolyte leakage rate of P. euphratica cells. Exogenous ABA (5 μmol·L-1) application could alleviate SO2-induced toxicity, decreasing the cell death rate and electrolyte leakage rate. Under 2 mmol·L-1 SO2 derivatives exposure, exogenous ABA enhanced the activity of ascorbate peroxidase (APX) and catalase (CAT) by 23.9% and 48.0%, compared with SO2 treatment alone, respectively, contributing to the scavenging of H2O2 within P. euphratica cells. Furthermore, exogenous ABA could remarkably inhibit PeNia1 and PeNia2 gene expression, and decrease nitrate reductase (NR) activity by 24.1%, thus restricted NO generation within P. euphratica cells. These results suggested that exogenously applied ABA could alleviate SO2-induced toxicity by reducing H2O2 and NO level in P. euphratica cells.

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